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i tried as follows dataGridView1.Columns.RemoveAt(12); i mentioned the index of checkbox and its worked. Thanks All exactly Mr jmcilhinney :-p , but i have to execute again and again with new inputs :-( this is the requirement :-) anyway i have solved this :-)

i have created datagridview dynamically with check box column,once i refresh my datagrid its removes all data except checkbox column,below code for adding checkbox column in datagrid DataGridViewCheckBoxColumn chk = new DataGridViewCheckBoxColumn(); dataGridView1.Columns.Add(chk)...